.xml metadata and pixel dimensions

[GaetanoLombardi]

Hi to everyone.

I have a lot of .tiff images from a confocal microscope. Each image has a .xml file. The tags included in these files are a little different from the standard OME (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175959/). 

I need to calibrate MCiD Analysis and set pixel dimensions to obtain correct measurements. I think the useful tags are 

- <Distance Id="X">
<Value>2.1972656236198625E-08</Value>
</Distance>

- <Distance Id="Y">
<Value>2.1972656236198625E-08</Value>
</Distance>

but I don't know how to interpret them (pixel per micron?; micron per pixel?) Can someone help me?

Thank you to everyone.

[sbesson]

Hi,

as you can see from this table, there are quite a few formats using a combination of XML and TIFF formats. Do you have more information about the nature of the microscope and software used for acquiring the images?
Have you tried opening your data using Bio-Formats to see if the fileset is detected as one of the existing file formats and the metadata is parsed?

Best,
Sebastien