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Problem opening certain .nd2 files in ImageJ

Historical discussions about the Bio-Formats library. Please look for and ask new questions at https://forum.image.sc/tags/bio-formats
Please note:
Historical discussions about the Bio-Formats library. Please look for and ask new questions at https://forum.image.sc/tags/bio-formats

If you are having trouble with image files, there is information about reporting bugs in the Bio-Formats documentation. Please send us the data and let us know what version of Bio-Formats you are using. For issues with your code, please provide a link to a public repository, ideally GitHub.

Problem opening certain .nd2 files in ImageJ

Postby Ylva » Fri Feb 01, 2013 4:12 pm

I'm had an annoying problem with ImageJ today, well, the problem is still there actually since I haven't been able to solve it. Maybe someone here can help me?

It's the following: I need to convert .nd2 files containing time measurements to a tiff stack for my image analysis program (= in house developed) to be able to process them. The easiest way is (supposed to be) using ImageJ. So far, it worked for half of my movies, but not for the other half. As far as I can tell, there is no difference in image acquisition or the way I save the images. The only difference is that for the movies that open fine in ImageJ, I used the 488nm laser and the part that gives an error and does not want to open in ImageJ was imaged using the 561 nm laser.

The error I get is:
java.lang.ArrayIndexOutOfBoundsException: 503
at loci.formats.in.NativeND2Reader.initFile(NativeND2Reader.java:533)
at loci.formats.FormatReader.setId(FormatReader.java:1320)
at loci.formats.DelegateReader.setId(DelegateReader.java:267)
at loci.plugins.in.ImportProcess.initializeFile(ImportProcess.java:482)
at loci.plugins.in.ImportProcess.execute(ImportProcess.java:146)
at loci.plugins.in.Importer.showDialogs(Importer.java:141)
at loci.plugins.in.Importer.run(Importer.java:79)
at loci.plugins.LociImporter.run(LociImporter.java:81)
at ij.IJ.runUserPlugIn(IJ.java:184)
at ij.IJ.runPlugIn(IJ.java:151)
at ij.IJ.runPlugIn(IJ.java:140)
at loci.plugins.shortcut.ShortcutPanel$1.run(ShortcutPanel.java:192)

The .nd2 files were recorded with a very recent version of NISElements (confocal). Can someone help me out on this? I really really need the tiff stacks, otherwise I cannot analyse my data...
Ylva
 
Posts: 2
Joined: Fri Feb 01, 2013 3:58 pm

Re: Problem opening certain .nd2 files in ImageJ

Postby pwalczysko » Mon Feb 04, 2013 10:00 am

Hello Ylva
Thank you for the post.
It looks like a Bioformat issue.
Would you be so kind please and upload some of the files you have problems with ?
Otherwise it would be difficult for us to assess exactly what the problem is.
Please use the QA system.
Go to
http://qa.openmicroscopy.org.uk/qa/upload/
and upload the images.
Thank you very much
All the best
Petr
User avatar
pwalczysko
Team Member
 
Posts: 11
Joined: Wed Oct 03, 2012 9:39 am

Re: Problem opening certain .nd2 files in ImageJ

Postby Ylva » Tue Feb 05, 2013 8:14 am

Hi Petr,

I uploaded a file (FRAP1.nd2). I hope this problem can be solved. Please, let me know if you need any more information.
Ylva
 
Posts: 2
Joined: Fri Feb 01, 2013 3:58 pm

Re: Problem opening certain .nd2 files in ImageJ

Postby pwalczysko » Tue Feb 05, 2013 9:35 am

Hi Ylva
thank you very much for the upload.
We will investigate and let you know.
All the best
Petr
User avatar
pwalczysko
Team Member
 
Posts: 11
Joined: Wed Oct 03, 2012 9:39 am


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