Open Microscopy Environment

Hi,

I'm trying to load Aperio SVS images into Octave. It's worked fine in the past using Matlab, but for deployment reasons I need to use Octave this time.

The initial problem is lack of inputParser in Octave, but that's fairly easy to get around by reducing the flexibility of the argument parsing in a couple of places. But then I hit problems with it failing to find 'loci' where the code tries to call version = char(loci.formats.FormatTools.VERSION); (but the latest version of Bio-formats isn't meant to require loci_tools.jar, not that downloading them makes any difference).

Comment that out, I hit problems with loci.common.DebugTools.enableLogging('INFO'). Again, commenting this out, I then hit:

error: 'java' undefined near line 36 column 11
error: called from:
error: C:\Temp\INSERM\bfmatlab\bfCheckJavaMemory.m at
error: C:\Temp\INSERM\bfmatlab\bfGetReader.m at line 5
error: C:\Temp\INSERM\bfmatlab\bfopen.m at line 114, c

which is runtime = java.lang.Runtime.getRuntime();

Java is definitely installed, so not sure where to go from here, any suggestions gratefully received.

Thanks
Phil

Hi Phil,

the code may indeed be a MATLAB specific implementation to retrieve the JVM memory settings. Browsing various pieces of code, could you try something like:

Code: Select all

runTime = javaMethod("getRuntime", "java.lang.Runtime");
maxMemory = runTime.maxMemory();


Best,
Sebastien

Hi Sebastien,

Thanks for the suggestion. That gets me a bit further, but now I'm hitting errors with the following:

r = loci.formats.ChannelFiller();
r = loci.formats.ChannelSeparator(r);
if stitchFiles
r = loci.formats.FileStitcher(r);
end

OMEXMLService = loci.formats.services.OMEXMLServiceImpl();

The error is:
error: 'loci' undefined near line 79 column 5

Any further suggestions?

Many thanks
Phil

Hi Phil,

at this point, it would be useful to know the following so that we can try to reproduce your steps:
- the OS you are using
- the version of Octave you installed
- the javaclasspath as returned by Octave when you receive these errors
- the modified scripts you use.

In all cases, bear in mind the bfmatlab zip file was not produced with Octave in mind and we don't have any infrastructure in place to test it. It might be compatible to a certain extent but it is hard to predict how much is required to adapt it fully to Octave.

Best regards,
Sebastien

Hi Sebastien,

Completely appreciate it's not produced with Octave in mind, just keen to get it working if turns out to be fairly straightforwards.

OS is Windows 7, Octave version is 3.8.1.

Javaclasspath returns:

STATIC JAVA PATH

- empty -

DYNAMIC JAVA PATH

C:\Temp\bfmatlab\bioformats_package.jar

That is the correct path to the jar file and I've double checked the file is there.

I've attached a zip of the scripts. I call it using Coloc('<path to test svs file>'). I've obviously deleted the jar file from bfmatlab subdirectory to reduce the attachment size.

Thanks for your help

Phil

Hi Phil,

first of all can you confirm this method:

Code: Select all

r = loci.formats.ChannelFiller();


returns without error but the following:

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OMEXMLService = loci.formats.services.OMEXMLServiceImpl();


generates an exception.

If this is the case, it may be that some JARs included in MATLAB are missing in Octave (since your static path is empty).

Apart from Bio-Formats classes, the following classes are imported in OMEXMLServiceImpl.java. Is there a way for you to check whether these classes are defined under Octave?

Code: Select all

import java.io.IOException;
import java.util.HashSet;
import java.util.Hashtable;
import java.util.List;
import java.util.Set;

import javax.xml.parsers.DocumentBuilder;
import javax.xml.parsers.DocumentBuilderFactory;
import javax.xml.parsers.ParserConfigurationException;
import javax.xml.transform.Templates;
import javax.xml.transform.TransformerConfigurationException;
import javax.xml.transform.TransformerException;

import org.slf4j.Logger;
import org.slf4j.LoggerFactory;
import org.w3c.dom.Document;
import org.w3c.dom.Element;
import org.w3c.dom.NamedNodeMap;
import org.w3c.dom.Node;
import org.w3c.dom.NodeList;
import org.xml.sax.SAXException;


Best,
Sebastien

For what is worth, there is a bioformats Octave package since version 5.1.4.

There was an announcement on the octave mailing list

https://lists.gnu.org/archive/html/help ... 00136.html

with the main captchas:

1. it is not an octave forge release so the -forge flag won't work
2. you need to install the jar file separately and add it to the static javaclasspath