CZI from Elyra PALM
Posted: Wed Jul 16, 2014 12:43 am
Hi
I have been using the 'Convert to Image' functionality of Zen (Black) to save STORM image data (reconstructed images) and I have noticed an issue opening the file saved as CZI using Bioformats (in FIJI and Omero). The two planes that get saved to the image have different XY dimensions:
HR: x: 1280, y: 1280
WF: x: 128, y: 128
And the file opens at the WF dimensions and as a result (I think) the pixel data in the HR plane is lost. I can export the data as tif but it would mean saving two files rather than one. I have uploaded an example.
Cheers,
Dan.
I have been using the 'Convert to Image' functionality of Zen (Black) to save STORM image data (reconstructed images) and I have noticed an issue opening the file saved as CZI using Bioformats (in FIJI and Omero). The two planes that get saved to the image have different XY dimensions:
HR: x: 1280, y: 1280
WF: x: 128, y: 128
And the file opens at the WF dimensions and as a result (I think) the pixel data in the HR plane is lost. I can export the data as tif but it would mean saving two files rather than one. I have uploaded an example.
Cheers,
Dan.