Open Microscopy Environment

Hi all,

I am working with loci_tools.jar 5.0.4 to open my ND2 files. These are typically TCZ stacks.
Mostly this works perfectly fine, only somtimes dimensions get scrambled around. I already wrote a workaround for this, but it would be great if this would be solved in bioformats. The problem is as follows:

T stacks are saved as multipoints and Z is recognized as T stacks. For example, a 512x512x2x100x40 XYCTZ gets recognized as a collection of 512x512x2x40x1 files. The imagecount in the collection is 100 in this example. I use the loci.formats.meta.MetadataRetrieve functions to get these sizes.

Moreover, the frames are not distributed in the way you would expect. Normally you would expect 3D frame 1 to be in the first 80 frames (2x40), with channel 0 in the even frames and channel 1 in the odd frames. If you readout loci.formats.IFormatReader.getIndex(z,c,t), considering Z as T and T as MP, you get this behavior. And also the MetadataRetrieve functions are OK.

But actually, frame 0, channel 0 is in the first frames of the first 40 multipoints. And channel 1 is in the first frames of the first 40 multipoints. I denote this (0-39,0) and (0-39,1), first is the image index, second the frame index.

3D Frame 0 has (0-39,0) and (0-39,1)
3D Frame 1 has (40-79,0) and (40-79,1)
3D Frame 2 has (80-99,0) + (0-19,2) and (80-99,1) + (0-19,3)
3D Frame 3 has (20-59,2) and (20-59,3)
etc.

So if you think about this, you can do:

Code: Select all

multipoint = (t*sizeT + z)%imageCount,
frame index = (t*sizeT)//imageCount + c


With % is the remainder operator and // the floor operator. This is how I work now. Because you keep changing between multipoints, the loading times get longer.

My smallest file with this error is 1.7GB, I can upload it if you want. If I crop it, the error goes away... I will also e-mail Nikon with this issue, but for now I wonder if there are people struggling with this too?

I think the file can be found here:

http://qa.openmicroscopy.org.uk/qa/feed ... a4d962e3c7

Thanks, I can reproduce this exactly as you have described, and I've opened a ticket for this here with you CCd on it:
https://trac.openmicroscopy.org.uk/ome/ticket/12592


Thanks,
Roger Leigh

caspervdw wrote:And also the MetadataRetrieve functions are OK.

I would like to comment on myself, the MetadataRetrieve functions are also not OK. Using the indices as described above, I cannot obtain correct timestamp values for example. Also using the (mp,z,c,t) indices with t=z and mp=t doesn't work. It seems like part of the metadata is not accessible, but I cannot say how it works exactly.

I would not expect this to behave sensibly until the ND2Reader is computing the dimensionality correctly, at which point the MetadataRetrieve interface should start returning correct values.