Open Microscopy Environment

Hi all,

I've been uploading EM files to OMERO and realised that OMERO does not appear to properly scale images for displaying when the file is signed byte. So everything that is below 0 gets truncated and its impossible to visualise the image properly in omero. Not sure if this is a bio-formats bug or just a display issue in OMERO so ended up posting here.

Uploaded file: image.mrc~
Cheers,
Helio

Hi Helio,

could you also tell us what version you are currently using?

Cheers,
~Josh

Hi Josh,
Sorry forgot about that info. Am using 5.0.8.
Helio

Hi Helio,

thanks for uploading your sample image. Afer verification, at the moment, the MRC reader does not support the mode 0 with signed bytes and forces unsigned bytes for this mode. So the truncation is happening directly at the Bio-Formats level.

I opened a Pull Request to add support for signed bytes following the MRC specification. I would expect this PR to be included as part of the 5.1.0 release.

Best,
Sebastien