.xml metadata and pixel dimensions
Posted: Tue Jan 24, 2017 10:23 am
Hi to everyone.
I have a lot of .tiff images from a confocal microscope. Each image has a .xml file. The tags included in these files are a little different from the standard OME (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175959/).
I need to calibrate MCiD Analysis and set pixel dimensions to obtain correct measurements. I think the useful tags are
but I don't know how to interpret them (pixel per micron?; micron per pixel?) Can someone help me?
Thank you to everyone.
I have a lot of .tiff images from a confocal microscope. Each image has a .xml file. The tags included in these files are a little different from the standard OME (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1175959/).
I need to calibrate MCiD Analysis and set pixel dimensions to obtain correct measurements. I think the useful tags are
- <Distance Id="X">
<Value>2.1972656236198625E-08</Value>
</Distance>
- <Distance Id="Y">
<Value>2.1972656236198625E-08</Value>
</Distance>
but I don't know how to interpret them (pixel per micron?; micron per pixel?) Can someone help me?
Thank you to everyone.