nd2 error - wrong number of channels

[dsudar]

Hi,
We started collecting large multi-location (700 locations) nd2 images in 4 color channels of printed cell-spot arrays. The images are about 1200 by 1200 pixels each so the total datasets are about 7 GB each. In NIS-Elements the files show to have 4 channels (correctly) but when reading them into either Fiji (latest) or OMERO 5.1.2, they get interpreted to have only 3 channels and the image is scrambled. I tried to make these datasets smaller in NIS-Elements in order to be able to upload one to the QA system but whenever I do so, the resulting smaller dataset works fine in Fiji. Do you have a place where I can upload a 7 GB file so you can see the problem?
Thanks,
- Damir

[mlinkert]

Hi Damir,

Thank you for the bug report. I'll send a private message with information on how to upload large files.

-Melissa

[dsudar]

Thanks Melissa, the file is now uploaded.
Cheers,
- Damir

[dsudar]

Hi Melissa,
Were you able to confirm the issue with the ND2 file I uploaded? And is there likely an easy fix?
Thanks,
- Damir

[mlinkert]

Hi Damir,

My apologies for the delay - yes, I can reproduce the problem. We're working on a fix now, and I'd expect the upcoming 5.1.3 release to resolve the problem.

Regards,
-Melissa