How do I append frames using bfsave() in Matlab?

[trichner]

Hello Curtis, LOCI, and community,

Can you help with the following? I would like to add frames to a tiff stack without loading the entire stack into memory. I'm working in Matlab, and I'm using BigTiffs. If appending isn't an option, should I just use individual tiffs for large data sets?

Great thanks for Bioformats--use it nearly every day. Thank you,
Tom

[trichner]

I found this thread: https://www.openmicroscopy.org/community/viewtopic.php?f=13&t=5812&p=11203&hilit=append#p11203

I'll give Matlab's tiff class a try and report back.

Thanks,
Tom

[i.munro]

Hi Tom

It's certainly possible to use Bio-Formats and the bfmatlab plugin to open a tiff and then add planes one-by-one (e.g. as they are acquired) before closing the file. if this is what you mean I can send some example Matlab code?

Best Wishes

Ian