Multiple excitation wavelengths

[Simon Carter]

Hi

I'm implementing some code to write OME meta data to tiff files captured by a device which has multiple light sources and thus excitation wavelengths for each collection channel. I can't see from the documentation at http://git.openmicroscopy.org/src/devel ... Wavelength how this might be captured in the xml.

Any suggestions?
In any case, should I be using the light source settings element to specify this rather than the excitation wavelength attribute?

Regards

[ajpatterson]

Hello Simon,

At present this is not possible. We have had this requested once before and I have updated that ticket to add a link to you post.
https://trac.openmicroscopy.org.uk/ome/ticket/3673

The way we can see this working is changing Chanel to allow multiple LightSourceSettings, at present you are limited to one. The wavelength would then be stored in the LightSource.

Unfortunately we are past the point where the model was frozen for the 4.4 release so this will not make it into 4.4.

You can have multiple ExcitationFilters in the LightPath. Each of these can have various transmittance wavelength values.
http://git.openmicroscopy.org/src/devel ... tanceRange

I know this is not right, but would this be any use to you?

Andrew

[Simon Carter]

Thanks for the advice. At the moment I'm second-guessing what our customers are going to need; I may postpone adding multiple excitation wavelengths to the metadata until it appears in the model rather than doing something non-standard now.